小福利导航

Analytical services: 未2贬 and 未18翱 in natural waters

The oxygen (未18翱) and hydrogen (未2贬) isotopic composition of water can be determined by isotopic equilibration of sample water with an appropriate gas mix in a headspace vial in a Gasbench II. Sample water is added to 12 ml exetainer headspace vials and then flushed with an appropriate gas mixture. A period of isotopic equilibration allows atoms to exchange with the headspace flush gas until the headspace gas has taken on the isotopic composition of the sample water (after  correcting for liquid-gas phase fractionation). Because the two elements need different equilibrations gases, two separate analyses must be performed to analyze both elements. The SPA lab can analyze the same aliquot of water for both analyses at a small loss of precision (mostly for 未2贬).  However, the small amount of water required for each analysis (200 渭L) usually allows for analysis of separate aliquots of water. After this equilibration period, the headspace gas is entrained into a He stream, water vapor is removed, passed through a gas chromatographic (GC) column and finally introduced into the MAT-253 mass spectrometer for isotopic analysis.

During analysis, two different calibrated in-house water standards are interspersed between samples to normalize and correct for possible drift during the analysis run. Two calibrated laboratory standards are run "as a sample" for quality control and to monitor long-term performance. We use a light in-house standard derived from winter precipitation and a heavier in-house standard derived from summer precipitation. These laboratory standards, which span most of the 未18翱 isotopic range found in nature, have been previously calibrated against NIST Standard Reference Materials (SMOW, SLAP, and GISP). We use a two-point linear normalization approach and report delta values relative to Standard Mean Ocean Water (SMOW).

未18翱 in natural waters

The 未18翱 value of water can be easily determined by equilibrating the water with a helium flush gas containing 3% CO₂. The vials are then allowed to equilibrate for 18 hours (usually overnight). Minimum sample size is 200 渭L.

未2贬 in natural waters

The 未18翱 value of water can be determined by equilibrating the water with a helium flush gas containing 2% H2.  A platinum catalyst is added to vial to facilitate isotopic exchange in the gas phase. Copper and charcoal can be added for increased precision should the researcher prefer, though we have not seen a large increase in precision for most natural waters with medium to low DOC. The vials are then allowed to equilibrate for 3 hours. Minimum sample size is 200 渭L.

未2贬 and 未18翱 on same water aliquot

Separate analyses are required to obtain both 未2贬 and 未18翱 on the same aliquot of water. First, the 未18翱 is determined per the above procedures. Next, the exetainer caps are removed, a platinum catalyst rod is added and vials are sealed with new caps, flushed and analyzed per the procedures for 未2贬 outlined above. The analyses take 3 days to complete. Minimum sample size is 200 渭L.

未2贬 and 未18翱 of water rates

Isotope	Phase	Quantity	Measurement Precision	Internal	External
未18翱	Liquid	0.2鈥�1 mL	卤 0.2 鈥�	$6	$10
未2贬	Liquid	0.2鈥�1 mL	卤 1 鈥�	$6	$10
未18翱 and 未2贬	Liquid	0.2鈥�1 mL	卤 0.2 and 卤3 鈥�	$11	$18

Sample types: natural waters

Sample requirements: Water samples for 未2贬 and/or 未18翱 should be submitted in vials with no head space. As noted above, we usually need at least 200 渭L for each analysis. For small sample amounts, we use 2 ml shorty vials with conical-bottomed caps. If you ship extracted water samples in glass tubes and need part of your samples to be saved, please provide us with appropriate vials for return shipment. While we can extract water from plant material using an extraction line, this is only practical for researchers very nearby to 小福利导航 College.